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KMID : 1094720120170020290
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 2 p.290 ~ p.297
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Fundamental analysis of real-time PCR quantification and modeling for thermophilic L-lactate fermentation by Bacillus coagulans from glucose
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Hidaka Taira
Tsuno Hiroshi
Yagi Haruka
Kosaka Yusuke
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Abstract
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Batch and semi-continuous thermophilic l-lactate fermentation experiments were performed using Bacillus coagulans and glucose as a substrate. Reactor performance and biomass concentrations were assessed using two methods: turbidity as a traditional biomass index and real-time polymerase chain reaction (PCR) quantification of 16S rRNA genes. In the batch experiment, although the relationship between turbidity and real-time PCR assay differed depending on the growth phase, a correlation was observed between both assay methods. In the semi-continuous experiment, real-time PCR measurement was well suited for use as an index for evaluating bacterial mass under different organic loading conditions. A mathematical model was applied to evaluate the real-time PCR quantification to long-term, semi-continuous lactate fermentation. Lactate fermentation was well suited since only B. coagulans was involved in the reactions. The results obtained revealed a fundamental relationship between real-time PCR and traditional biomass analyses.
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KEYWORD
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real-time PCR, Bacillus coagulans, lactate fermentation, turbidity, inhibition, glucose
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